How should euthanasia of research animals be performed according to AWA regulations?

Euthanasia of research animals must be performed humanely and in accordance with acceptable veterinary practices to ensure that the process minimizes pain and distress for the animals involved. This aligns with the core principles of the Animal Welfare Act (AWA), which emphasizes the humane treatment of animals in research settings.

Acceptable veterinary practices refer to established guidelines and protocols that have been vetted by veterinary professionals and organizations, ensuring that the methods used are scientifically validated and ethically sound. This ensures not only the welfare of the animals but also the integrity of the research being conducted.

Methods that do not prioritize humane treatment, such as those implied in the other choices, are not consistent with AWA regulations. For instance, "politely and quietly" does not address the necessity for scientifically appropriate and humane methods. "By any means necessary" and "at the discretion of the researcher" suggest a lack of regulation and oversight that could lead to inhumane treatment or practices that are not recognized as valid within veterinary standards. Therefore, the requirement for humane euthanasia practices is crucial for compliance with the AWA and reflects a commitment to ethical research.